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5-Methylcytosine Antibody

5-Methylcytosine Antibody

Item no.	AMM99021
Manufacturer	AVIVA Systems Biology
Amount	50 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot ELISA Immunofluorescence Immunocytochemistry Immunoassay Primary Antibodies CHiP Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IP, IHC, ICC, CHIP, IA
Clone	33D3
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Chicken (Gallus gallus domesticus), Dog (Canine, Canis lupus familiaris), Pig (Porcine, Sus scrofa domesticus), Zebrafish (Danio rerio)
Host	Mouse
Isotype	IgG1
Purity	> 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)
Citations	Conformation-selective methylation of geminivirus DNA. J Virol. 85, 12001-12 (2011) 21835804 Dynamic heterogeneity of DNA methylation and hydroxymethylation in embryonic stem cell populations captured by single-cell 3D high-content analysis. Exp Cell Res. 332, 190-201 (2015). 25700729 Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446-54 (2013). 23739127 Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). 23394161 Global acetylation and methylation changes predict papillary urothelial neoplasia of low malignant potential recurrence: a quantitative analysis. Int J Immunopathol Pharmacol. 24, 489-97 (2011) 21658323 Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500-8 (2008). 18448484 Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533-8 (2012). 22880797 Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799-803 (2012). 22263690 Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722-35 (2011). 21874386 Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780-7 (2008). 17936611 Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays. BMC Mol Biol. 12, 49 (2011). 22098709 Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115-21 (2009). 18752633 Physical localization and DNA methylation of 45S rRNA gene loci in Jatropha curcas L. PLoS One. 8, e84284 (2014). 24386362 PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 27, 46-56 (2018). 29324254 Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports. 9, 329-341 (2017). 28579394 Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers. Oncotarget. 8, 57278-57301 (2017). 28915670 Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372-80 (2011). 21497756 Single cell epigenetic visualization assay. Nucleic Acids Res. (2021). 33511400 Siyuan Chang, et al. Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment. 452 (2020). 32944000 Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77-88 (2013). 23592032 The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice. EMBO J. 34, 748-58 (2015). 25519955 Utility of 5-Methylcytosine Immunohistochemical Staining to Assess Global DNA Methylation and Its Prognostic Impact in MDS Patients. Asian Pac J Cancer Prev. 18, 3307-3313 (2017). 29286224 Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). 18922183 Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215-28 (2010). 20482652 Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25-34 (2008). 18239133 Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938-43 (2008). 18593814 Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008). Walsh, T. K., et al., Insect Mol Biol. 19 Suppl 2:215-28 (2010). Brena, R. M., et al., Methods in Molecular Biology, 507, Part IV, 257-269 (2009). Zhang, W., et al., Plant Cell. 20(1):25-34 (2008). Zhang, W., et al, Genome Res. 18(12):1938-1943 (2008). Jensen, T. J., et al., Carcinogenesis.29(8):1500-1508 (2008). Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008). Vrba, L., et al., BMC Genomics. 15;9:486 (2008). Vinsky, M. D., et al., Reprod Fertil Dev. 19(5):603-611 (2007). Novak, P., et al., Cancer Res. 66(22):10664-10670 (2006).
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Type	Monoclonal Antibody
Manufacturer - Applications	FC, IHC-FP, ICC, IP, CHIP, RIA, IF
Manufacturer - Category	Root Catalog/Products/Monoclonal Antibodies, Root Catalog/Products/Monoclonal Antibodies/Transcription Regulation, Root Catalog/Research Areas/Cell Biology, Root Catalog/Research Areas/Chromatin & Nuclear Signaling, Root Catalog/Research Areas/Developmental Biology, Root Catalog/Research Areas/Epigenetics, Root Catalog/Research Areas/Apoptosis, Root Catalog/Research Areas/Disease Related, Root Catalog/Research Areas/Meiosis\/Mitosis\/Cell Cycle, Root Catalog/Research Areas/Flow Cytometry Antibodies, Root Catalog/Products/Primary Antibodies
Shipping Temperature	Wet Ice
Product format	Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.
Reconstitution and storage	Ready for usage. For longer periods of storage, store at -20C. Can be stored 1 month at 4C.Avoid repeat freeze-thaw cycles.
Description of target	Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
Clonality	Monoclonal
Purification	Protein A ceramic hyper DF affinity chromatography
Concentration	1 mg/ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ug

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Delivery expected until 10/30/2025

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