HRH1 antibody - C-terminal region

Root Catalog/Products/Polyclonal Antibodies, Root Catalog/Research Areas/Immunohistochemistry, Root Catalog/Research Areas/GPCR, Root Catalog/Products/Primary Antibodies

55, 8

Peptide ELISA: Antibody detection limit dilution 1:32, 000.
Western Blot: Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3 ug/ml antibody staining. Please note that an explanation cannot be currently found in the literature for the 37 kDa band observed given the calculated size of 55.8 kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).
IHC: In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6 ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5 ug/ml. This product has been successfully used in IHC on Rat
IF: This product has been successfully used in IF on Rat
Optimal dilutions should be determined by the end user.

histamine receptor H 1|histamine receptor H1

Aliquot and store at -20°C. Minimize freezing and thawing.

Histamine H1 receptor

Ammonium Sulfate Precipitated; Affinity Purified

Human, Mouse, Rat, Dog, Cow

0.5 mg/ml