ADORA2A Antibody

FC, IHC, IHC-P, IHC-PFA, WB

Wet Ice

Immunohistochemistry: OALA02443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02443 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.

ADORA2A

Short term: store at 4C. Long term: aliquot and store at -20C. Avoid freeze-thaw cycles.

NP_000666.2

Protein G purified

For research use only.

0.5 mg/ml

0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide