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RB1 Antibody (Phospho-Thr826)

RB1 Antibody (Phospho-Thr826)

Item no.	OAAF00204
Manufacturer	AVIVA Systems Biology
Amount	100 ug
Category	Oncology Neuroscience Neural Lineage Markers Neural Transcription Factors Aging & Neurological Disorders Neural Signaling Neural Epigenetics Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Status Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Senescence Primary Antibodies Astrocytoma By Organ Bladder Cancer Immunostaining Bone Cancer Neuronal Cell Markers Immunochemistry
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, IHC, ICC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Citations	Lee W.-H., Nature 329:642-645(1987). Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987). McGee T.L., Gene 80:119-128(1989).
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	exon 17 tumor GOS561 substitution mutation causes premature stop;GOS563 exon 17 substitution mutation causes premature stop;OSRC;p105-Rb;p110-RB1;pp110;PPP1R130;pRb;prepro-retinoblastoma-associated protein;protein phosphatase 1,regulatory subunit 130;RB;retinoblastoma 1;retinoblastoma suspectibility protein;retinoblastoma-associated protein.
Shipping Condition	Cool pack
Available
Manufacturer - Type	Polyclonal Antibody
Manufacturer - Applications	Enzyme-linked immunosorbent assay\|Immunocytochemistry\|Immunofluorescence\|Immunohistochemistry\|Immunohistochemistry-Paraffin\|Western blot
Manufacturer - Category	Root Catalog/Products/Polyclonal Antibodies, Root Catalog/Products/Polyclonal Antibodies/Phospho Specific, Root Catalog/Products/Primary Antibodies, Root Catalog/Research Areas/Cancer/Cancer Cell Cycle, Root Catalog/Research Areas/Cancer/Cancer Signal Transduction, Root Catalog/Research Areas/Cancer/Cancer Transcription Factor
Shipping Temperature	Wet Ice
Molecular Weight	106 kDa
Manufacturer - Application Additional Information	WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:1000
Gene symbol	RB1
Gene Fullname	RB transcriptional corepressor 1
Product format	Liquid
Reconstitution and storage	-20°C
Description of target	Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Protein accession_num	NP_000312.2
Protein name	Retinoblastoma-associated protein
Clonality	Polyclonal
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen	The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr826.
Dilution	The optimal working dilution should be determined by the end user.
Manufacturer - Specificity	Retinoblastoma (Phospho-Thr826) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at threonine 826.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/11/2025

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