Anti-GAP-43

Polyclonal Antibody

Wet Ice

WB: 1:2000
IF: 1:500

Liquid. Total IgY fraction in PBS + 10mM NaN₃. Adequate amount of material to conduct 10-mini Western blots

GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Neuromodulin

Total IgY fraction.

Specific for the ~43k GAP-43 protein. The antibody has been directly tested for reactivity in Western blots with human, mouse, and rat tissue.