ITGB4 Antibody

Monoclonal Antibody

Wet Ice

This antibody is for use in Western Blot only under non-reducing conditions. Immunohistochemistry: OALA03573 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA03573 was determined to be 20 ug/ml. Tested by flow cytometric analysis of A549 cell line. Antibody can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

ITGB4

Store at 4C, avoid freeze-thaw cycles.

NP_000204.3

Affinity purified

For research use only.

Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.. Sourced from tissue culture supernatant