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CD8A Antibody

CD8A Antibody

Item no.	OAEE00331
Manufacturer	AVIVA Systems Biology
Amount	0.1 mg (C100)
Category	Oncology Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Adenocarcinoma Flow Cytometry Immunohistochemistry Immunoprecipitation Immunocytochemistry Primary Antibodies Immunochemistry Functional Assays
Type	Antibody Monoclonal
Applications	FC, IP, IHC, ICC, FA
Clone	53-6.7
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2a Kappa
Purity	> 95% (by SDS-PAGE)
Citations	Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterialpathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5102-7. Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177(1):306-14. Ko SY, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309-17. Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, SunderkÃ¶tter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109(2):183-92. Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114(4):582-8. Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5557-61. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95. Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	BB154331;Ly-;Ly-2;Ly-3;Ly-35;Ly-B;Lyt-;Lyt-2;Lyt-2.1 lymphocyte differentiation antigen (AA at 100);T-cell surface glycoprotein CD8 alpha chain;T-cell surface glycoprotein Lyt-2.
Similar products	CD8a
Shipping Condition	Cool pack
Available
Manufacturer - Type	Monoclonal Antibody
Manufacturer - Applications	Flow cytometry\|Functional assay\|Immunocytochemistry\|Immunohistochemistry-Frozen\|Immunohistochemistry-Paraffin\|Immunoprecipitation
Manufacturer - Category	Root Catalog/Products/Monoclonal Antibodies, Root Catalog/Products/Primary Antibodies
Shipping Temperature	Wet Ice
Manufacturer - Application Additional Information	Functional application: Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation. Flow cytometry: Recommended dilution: 1.5 ug/ml. Immunohistochemistry (frozen sections): Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.
Gene symbol	Cd8a
Gene Fullname	CD8 antigen, alpha chain
Product format	Phosphate buffered saline (PBS), pH 7.4
Reconstitution and storage	2°C to 8°C
Description of target	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Nucleotide accession_num	NM_001145873.1; NM_001768.6; NM_171827.3
Protein accession_num	NP_001139345.1; NP_001759.3; NP_741969.1
Protein name	T-cell surface glycoprotein CD8 alpha chain
Clonality	Monoclonal
Purification	Purified
Immunogen	Murine spleen cells
Manufacturer - Specificity	The rat monoclonal antibody 53-6.7 recognizes murine CD8a (32-34 kDa; alpha chain of the CD8 antigen).
Formulation	Azide free
Concentration	1 mg/ml
Storage Buffer	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg (C100)

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