Anti-FLAG tag Affinity Gel

Monoclonal Antibody

Wet Ice

IP: 10 uL resin binds >1 ug FLAG fusion protein
Anti-DYKDDDDK Affinity Gel is optimally suited for immunoprecipitation and purification of FLAG tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide for competitive elution to recover fusion protein

2°C to 8°C

Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.

0.99 mg antibody per cc agarose