HISTIDINE TAG Antibody

Root Catalog/Products/Monoclonal Antibodies, Root Catalog/Research Areas/Flow Cytometry Antibodies, Root Catalog/Products/Primary Antibodies

Western Blotting: 1/500-1/1000

-20°C

Monoclonal

PAX6 transcription factor linked to histidine tag.

Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing
histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteinsexpressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian
(Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.In Western blotting of bacterial extracts the antibody has been shown not to cross-react with
any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.