Colon Membrane Lysate (0.1 mg)

Mouse colon tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚, C. The mouse colon tissue membrane is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue membrane protein is then Western analyzed by either GAPDH or β, -actin antibody, and the expression level is consistent with each lot.

Colon membrane lysate is for use in Western blotting, 10 μ, g to 20 μ, g per lane is recommended for mini gel. (Optimal dilution should be determined by user.)