Myelin Basic Protein Antibody ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

myelin basic protein

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

Myelin basic protein

Component: Amount
Myelin Basic Protein antibody Microplate: 96 Wells (12 x 8 Well strips)
Myelin Basic Protein antibody Lyophilized Standard: 2 x XXX
100X Detector Antibody: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
Stop Solution: 1 x 6 mL
TMB Substrate: 1 x 9 mL

2h, 30min

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myelin Basic Protein Antibody (Anti-MBP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Myelin Basic Protein Antibody (Anti-MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myelin Basic Protein Antibody (Anti-MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myelin Basic Protein Antibody (Anti-MBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%