Mix-n-Stain Kappa CF Kappa 680R Antibody Labeling Kit,1x(20-50ug) labeling (OKBE00051)

Mix-n-Stain™, antibody labeling kits contain everything you need to rapidly label an antibody with Aviva's next-generation CF™, dyes or biotin. The labeling procedure comprises simple mixing of your antibody with the reaction buffer and optimally formulated dye provided, followed by a brief incubation. The Mix-n-Stain dye is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. After labeling, the dye is covalently linked to the antibody with a degree of labeling of approximately 4-6 dye molecules per antibody molecule. Mix-n-Stain labeling is covalent, so Mix-n-Stain-labeled antibodies can be used for multicolor fluorescence staining without transfer of dyes between antibodies. Mix-n-Stain labeling can tolerate sodium azide, and low levels of glycerol, Tris, and glycine. A microcentrifuge ultrafiltration vial is provided in the kit to rapidly remove incompatible small molecule antibody stabilizers before labeling. The standard Mix-n-Stain labeling protocol can be performed in the presence of up to four-fold excess of BSA or gelatin to IgG (by ug amount). Simply choose the kit size that corresponds to the amount of IgG you wish to label. A modified protocol is provided for labeling IgG in the presence of excess stabilizer protein or ascites fluid. In this case, choose the kit size that corresponds to the total amount of protein (IgG + stabilizer, or total protein amount in ascites fluid) in the antibody sample you wish to label. The modified protocol also can be used to label amounts of IgG that fall below the lower range of the kit by adding stabilizer protein to the IgG to bring the total protein amount within the kit range. The modified protocol is not recommended for labeling antibodies in crude antiserum or hybridoma cell culture supernatant due to the low concentration of antibody relative to total protein in these formats. When performing direct immunofluorescence with a fluorescently-labeled antibody, you may need to use a higher concentration of antibody to achieve similar staining intensity compared to indirect immunofluorescence detection using unlabeled primary plus labeled secondary antibody. In our internal testing, indirect immunofluorescence staining results in about 3-fold signal amplification compared to direct immunofluorescence staining. Labeled secondary antibodies will still bind to primary antibodies labeled using Mix-n-Stain kits, therefore if multiple primary antibodies from the same species are to be used for multicolor immunofluorescence staining, a secondary antibody cannot be used to distinguish an unlabeled primary antibody from a Mix-n-Stain labeled primary antibody from the same species. Mix-n-Stain labeled antibodies can be used as a tertiary staining antibody after standard immunofluorescence staining with primary and secondary antibodies. Aviva also offers biotin Mix-n-Stain labeling kits, for secondary detection using CF™, dye-labeled streptavidin or CF™, dye-labeled monoclonal mouse anti-biotin.