T4 DNA Ligase Recombinant (OPPA00808)

Sterile filtered liquid formulation having a concentration of 167, 000 U/ml.

Recombinant T4 DNA Ligase

One Weiss unit is equivalent to circa 67 cohesive-end ligation units.
T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.
Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50uM.
To dilute T4 DNA Ligase that will subsequently be stored at -20C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.