MMP-2 Protein (OPPB00051)

Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-2 is a secreted collagenase with specificity toward Type IV, V, VII, and X collagens. Recombinant human MMP-2 is a 62.0 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (552 amino acids).

Preparation: MMP - 2 activity was measured by its ability to cleave a chromogenic peptide MMP - 2 substrate at room temperature. At an MMP - 2 concentration of 2.5 ug/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes.Storage: The lyophilized MMP-2 recombinant protein is stable for at least 2 years from date of receipt at -20C. Reconstituted MMP-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20C. As with any protein, exposing MMP-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.