TRAIL Protein (OPPB00199)

TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic 'death domain', which activates the cell's apoptotic machinery. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.

Preparation: Biological activity was determined by its ability to induce apoptotic cell death in TRAIL - sensitive U343MG cells. The expected ED50 for this effect is 1.0 - 3.0 ng/ml.Storage: The lyophilized TRAIL recombinant protein is stable for at least 2 years from date of receipt at -20C. Reconstituted TRAIL is stable for at least 3 months when stored in working aliquots with a carrier protein at -20C. As with any protein, exposing TRAIL recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.