Human beta-CTx (Beta Crosslaps) ELISA Kit

beta-CTx

125-8000 pg/mL

Colorimetric

Competitive

Short term: 4C; Long term: see manual.

6 months

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human beta-CTx. During the reaction, Human beta-CTx in the sample or standard competes with a fixed amount of Human beta-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human beta-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human beta-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.

The kit is manufactured at ISO 9001 certified facilities.