Rat hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit

hs-CRP

0.16-10 ng/mL

Colorimetric

Sandwich

Short term: 4C; Long term: see manual.

6 months

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat hs-CRP. The concentration of Rat hs-CRP in samples can be calculated by comparing the OD of the samples to the standard curve.

The kit is manufactured at ISO 9001 certified facilities.