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Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit

Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit

Manufacturer	Biomatik
Category	Cell Biology Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay Microfilaments
Type	Elisa-Kit
Specific against	Rat
Format	96-Well Plate
Amount	96T
Item no.	BM-EKU07912
eClass 6.1	32160605
eClass 9.0	32160605
Available
Estimated Turnaround Time	6-11 Business days
Target Name	Troponin T Type 2, Cardiac
Target Synonyms	CMH2, cTnT, TnTc, Cardiomyopathy, Hypertrophic 2, Cardiac muscle troponin T
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range	15.6-1000 pg/mL
Sensitivity	5.5 pg/mL
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 2, Cardiac were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 2, Cardiac were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
Detection Method	Colorimetric
Assay Time	3 hours
Assay Type	Double-antibody Sandwich
Shipping Condition	Ice packs
Storage	Short term: 4C; Long term: see manual.
Precaution of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date	8 months
UniProt ID	P50753
Specificity	This assay has high sensitivity and excellent specificity for detection of Troponin T Type 2, Cardiac
Cross Activity	No significant cross-reactivity or interference between Troponin T Type 2, Cardiac and analogues was observed.
Test Principle	No significant cross-reactivity or interference between Troponin T Type 2, Cardiac and analogues was observed.
Assay Procedure	1.Prepare all reagents, samples and standards; 2.Add 100 uL standard or sample to each well. Incubate 2 hours at 37C; 3.Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37C; 4.Aspirate and wash 3 times; 5.Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37C; 6.Aspirate and wash 5 times; 7.Add 90 uL Substrate Solution. Incubate 10-20 minutes at 37C; 8.Add 50 uL Stop Solution. Read at 450 nm immediately.
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance	Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.
Research Area	Cardiovascular biology

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96T

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