Rat Fibroblast Growth Factor 15 (FGF15) ELISA Kit

12.5-200 pg/mL

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Q8VI81

Fibroblast Growth Factor 15

Colorimetric

-20C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

8 months

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 15 (FGF15) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 15 (FGF15) and unlabeled Fibroblast Growth Factor 15 (FGF15) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 15 (FGF15). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 15 (FGF15) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 15 (FGF15) in the sample.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.