Mouse monoclonal antibody to human p75NTR [MLR2]: IgG2a

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer, disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.

After reconstitution keep aliquots at -20AC for a higher stability, and at 4AC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Lyophilised from PBS pH 7.4

This antibody is known to react with human, mouse, rat and guinea-pig p75NTR protein.

Specificity has been confirmed using a number of techniques as described in the reference by Rogers et al (2006). The antibody recognizes extracellular p75 in native configurations.

Panni P, Ferguson IA, Beacham I, Mackay-Sim A, Ekberg JA, John JA. (2013) "Phagocytosis of bacteria by olfactory ensheathing cells and Schwann cells" Neurosci Lett. 2013 Feb 13. pii: S0304-3940(13)00109-2. Application: Immunopanning, species: Mouse

Zhang C. et al (2012) Suppression of p75 neurotrophin receptor surface expression with intrabodies influences Bcl-xL mRNA expression and neurite outgrowth in PC12 cells PLoS One. 2012, 7(1)

Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush RA & Sendtner M (2009) Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010, 5(1):31-8.

Chacón PJ, ArÃvalo MA, TÃbar AR. (2010) NGF-activated protein tyrosine phosphatase 1B mediates the phosphorylation and degradation of I-kappa-Balpha coupled to NF-kappa-B activation, thereby controlling dendrite morphology. Mol Cell Neurosci. 2010 Apr, 43(4):384-93.

Selvaraj BT et al (2012) Local axonal function of STAT3 rescues axon degeneration in the pmn model of motoneuron disease. J Cell Biol. 2012 Oct 29, 199(3):437-51.

Rogers ML et al (2010) ProNGF mediates death of Natural Killer cells through activation of the p75NTR-sortilin complex. J Neuroimmunol. 2010 Sep 14, 226(1-2):93-103.