BioTeZ Polystreptavidin R Coating Kit (various surfaces)

Streptavidin binds very tightly to the small molecule Biotin. Streptavidin coating of solid phases offers a universal immobilization principle for the detection and analysis of proteins, peptides, PCR-fragments, haptens etc., which must be present in biotinylat-ed form. As a further development BioTeZ offers a polymerized Streptavidin, the Polystreptavi-din R, characterized by an extraordinary high Biotin binding capacity. Coatings made of Polystrept R combine the excellent binding capacity with a high chemo and thermo stability. It is very suitable for coating membranes, beads, biochips, plastics etc.
Polystreptavidin R is part of this set of reagents, which has been supplemented by a special solution for pre-coating. The additional pre-coating serves to improve the out-come and should be used especially for very inert surfaces to ensure a high quality coating with Polystreptavidin R. The coating procedure starts with the pre-coating step. The kit is used for adsorptive coating microplates, beads, membranes etc. made of metal or plastics like Polystyrene, Polyethylene, Polypropylene, Polycarbonate, Poly(methyl methacry-late) PMMA or Polyamides with Polystreptavidin "Maximum Capacity".

all immuno assays

Biotin

12 month

If applicable mentioned in the product data sheet

37C

adsorptive coating of Polystreptavidin

Pipettes or pipetting equipment, vials for dilution, distilled or deionised water