Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IF, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS1168-50 |
Targets |
HDAC7;;HDAC9;;MEF2A |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
Q9UKV0 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
HDAC9 (P1047) pAb detects endogenous levels of HDAC9 protein. |
WB |
Western blot (WB) analysis of HDAC 9 (P1047) pAb at 1:500 dilution Lane1:LO2 cell lysate Lane2:Mouse heart tissue lysate Lane3:Rat heart tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of HDAC 9 (P1047) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 111, 160 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Histone deacetylase 9, Short name=HD9, Histone deacetylase 7B, Short name=HD7, Short name=HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR |
Immunogen |
Synthetic peptide, corresponding to the C-terminual of Human HDAC 9. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.