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Cytokeratin 10 (Y160) polyclonal antibody

Cytokeratin 10 (Y160) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Neural Lineage Markers Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Intermediate filaments
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IF, IHC
Amount	100ul
Host	Rabbit
Item no.	BS1199-100
Targets	KRT10
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P13645
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200
Background	Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	Cytokeratin 10 (Y160) pAb detects endogenous levels of Cytokeratin 10 protein.
WB	Western blot (WB) analysis of Cytokeratin 10 (Y160) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:NIH-3T3 cell lysate Lane4:Rat brain tissue lysate
IHC	Immunohistochemistry (IHC) analyzes of Cytokeratin 10 (Y160) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
BiowMW	ca. 59 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	Keratin, type I cytoskeletal 10, Cytokeratin-10, Short name=CK-10, Keratin-10, Short name=K10, KRT10, KPP, CK-10, CK 10, CK10, Keratin 10, Keratin10
Immunogen	Synthetic peptide, corresponding to amino acids 130-180 of Human Cytokeratin 10.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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