Cytokeratin 17 (V412) polyclonal antibody

Q04695

Cytokeratin 17 is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Cytokeratin 17 contains 432 amino acids and is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Cytokeratin 17 functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3-sigma to mediate protein synthesis. Mutations in the gene encoding for Cytokeratin 17 lead to depressed protein translation and smaller sized skin keratinocytes, corresponding to decreased Akt/mTOR signaling activity. Cytokeratin 17 may be a useful marker for cervical stem cell identification, squamous cell carcinoma of the larynx, respiratory syncytial virus and transitional cell carcinomas of the human urinary tract.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Keratin 17/36(V412) pAb detects endogenous levels of Cytokeratin 17 protein.

Immunohistochemistry (IHC) analyzes of Cytokeratin 17 (V412) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

For research use only, not for use in diagnostic procedure.

Synthetic peptide, corresponding to amino acids 400-450 of Human Cytokeratin 17.