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Cytokeratin 20 (D409) polyclonal antibody

Cytokeratin 20 (D409) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Intermediate filaments
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IF, IHC
Amount	100ul
Host	Rabbit
Item no.	BS1207-100
Targets	KRT20
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P35900
Application all	WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200
Background	Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	Cytokeratin 20 (D409) pAb detects endogenous levels of Cytokeratin 20 protein.
WB	Western blot (WB) analysis of Cytokeratin 20 (D409) pAb at 1:500 dilution Lane1:A549 cell lysate Lane2:SP2/0 cell lysate Lane3:Rat kidney tissue lysate Lane4:Mouse kidney tissue lysate
IHC	Immunohistochemistry (IHC) analyzes of Cytokeratin 20 (D409) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
BiowMW	ca. 48 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	Keratin, type I cytoskeletal 20, Cytokeratin-20, Short name=CK-20, Keratin-20, Short name=K20, Protein IT, KRT20, CK 20, CK20, Keratin 20
Immunogen	Synthetic peptide, corresponding to amino acids C-terminus of Human Cytokeratin 20.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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