Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IF, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS1207-50 |
Targets |
KRT20 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
P35900 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 IF: 1:50ca.1:200 |
Background |
Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
Cytokeratin 20 (D409) pAb detects endogenous levels of Cytokeratin 20 protein. |
WB |
Western blot (WB) analysis of Cytokeratin 20 (D409) pAb at 1:500 dilution Lane1:A549 cell lysate Lane2:SP2/0 cell lysate Lane3:Rat kidney tissue lysate Lane4:Mouse kidney tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of Cytokeratin 20 (D409) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 48 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Keratin, type I cytoskeletal 20, Cytokeratin-20, Short name=CK-20, Keratin-20, Short name=K20, Protein IT, KRT20, CK 20, CK20, Keratin 20 |
Immunogen |
Synthetic peptide, corresponding to amino acids C-terminus of Human Cytokeratin 20. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.