Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IHC |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BS1644-100 |
Targets |
FN1 ;;NECTIN1 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
P02751 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
Fibronectin 1 (F2372) pAb detects endogenous levels of Fibronectin 1 protein. |
WB |
Western blot (WB) analysis of Fibronectin 1 (F2372) pAb at 1:500 dilution Lane1:Hela cell lysate Lane2:SP2/0 cell lysate Lane3:Rat lung tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of Fibronectin 1 (F2372) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. |
BiowMW |
ca. 260 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Fibronectin, Cold-insoluble globulin, Short name=CIG, Cleaved into the following 4 chains: Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, FN1, FN |
Immunogen |
Synthetic peptide, corresponding to amino acids 2331-2380 of Human Fibronectin 1. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.