Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IHC |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BS2377-100 |
Targets |
CHKA;;CHKB |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
Q9Y259 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase, the initial enzyme in the sequence, plays a role in cell growth proliferation. A related protein, ChoKB (also, known as choline kinase beta), is a 395 amino acid enzyme that catalyzes the phosphorylation of choline by ATP in the presence of magnesium, thereby yielding phosphocholine and ADP. Like all choline kinases, ChoKB possesses ethanoalamine kinase activity and catalyzes the phosphorylation of ethanolamine. The gene encoding ChoKB is located less than 1 kb upstream of the CPT1B gene, suggesting that the ChoKB gene may regulate transcription CPT1B. In mice, mutations and/or deletions in the gene encoding ChoKB are the cause of hindlimb muscular dystrophy and neonatal bone deformity. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
ChoKB (E324) pAb detects endogenous levels of ChoKB protein. |
WB |
Western blot (WB) analysis of ChoKB (E324) pAb at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat liver tissue lysate |
IHC |
Immunohistochemistry (IHC) analyzes of ChoKB (E324) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50. |
BiowMW |
ca. 45 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Choline/ethanolamine kinase, Choline kinase beta, Short name=CK, CKB, Choline kinase-like protein, Ethanolamine kinase, Short name=EK, Ethanolamine kinase beta, EKB, choline/ethanolamine kinase beta, CKEKB, CHKB, CHETK, CHKL |
Immunogen |
Synthetic peptide, corresponding to amino acids 300-342 of Human ChoKB. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.