Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS3487-50 |
Targets |
MAP2 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
P11137 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
MAP-2 (W14) pAb detects endogenous levels of MAP-2 protein. |
WB |
Western blot (WB) analysis of MAP-2 (W14) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of MAP-2 (W14) pAb in paraffin-embedded human brain tissue. |
BiowMW |
ca. 200 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Microtubule-associated protein 2, MAP2 |
Immunogen |
Synthetic peptide, corresponding to the N-terminus of Human MAP-2. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.