Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Applications |
WB, IHC |
Amount |
50ul |
Host |
Rabbit |
Item no. |
BS4259-50 |
Targets |
ADRA1D |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
P05023 |
Application all |
WB: 1:500ca.1:1000 IHC: 1:50ca.1:200 |
Background |
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle. |
Product |
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
Purification and Purity |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
Storage and Stability |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Specificity |
p-Na+/K+-ATPase alpha1 (S16) pAb detects endogenous levels of Na+/K+-ATPase alpha1 protein only when phosphorylated at Ser16. |
WB |
Western blot (WB) analysis of p-Na+/K+-ATPase alpha1 (S16) pAb at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Hela cell lysate Lane3:Raw264.7 cell lysate |
IHC |
Immunohistochemistry (IHC) analyzes of p-Na+/K+-ATPase alpha1 (S16) pAb in paraffin-embedded human breast carcinoma tissue at 1:50. |
BiowMW |
ca. 112 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Alternative Name |
Sodium/potassium-transporting ATPase subunit alpha-1, Short name=Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1 |
Immunogen |
Synthetic phosphopeptide derived from human Na+/K+-ATPase alpha1 around the phosphorylation site of Serine 16. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.