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MYL9 (phospho-S19) polyclonal antibody

MYL9 (phospho-S19) polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Cell Biology Cell Status Necrosis Cytoskeleton Western Blot Motor proteins Primary Antibodies
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB
Amount	100ul
Host	Rabbit
Item no.	BS4845-100
Targets	MYH14
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P24844/P19105
Application all	WB: 1:500ca.1:1000
Background	Myosin regulatory light chains, including MRCL3 (also known as MRLC3 or MLCB), MRLC2 (also known as MLC-B) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined.
Product	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	p-MYL9 (S19) pAb detects endogenous levels of MYL9 protein only when phosphorylated at Ser19. This antibody may detects endogenous levels of MYL12A protein only when phosphorylated at Ser18.
WB	Western blot (WB) analysis of p-MYL9 (S19) pAb at 1:500 dilution Lane1:LO2 cell lysate treated with EGF(EGF, 0.1ng/ML, 30min) Lane2:Raw264.7 cell lysate treated with EGF(EGF, 0.1ng/ML, 30min) Lane3:Rat heart tissue lysate
BiowMW	ca. 20 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, Short name=LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MLC2, MRLC1, MYRL2, Myosin regulatory light chain 12A, MLC-2B, Myosin RLC, Myosin regulatory light chain 2, nonsarcomeric, Myosin regulatory light chain MRLC3, MYL12A, MLCB, MRLC3, RLC
Immunogen	Synthetic phosphopeptide derived from human MYL9 around the phosphorylation site of Serine 19.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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