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Cytokeratin 8 polyclonal antibody

Cytokeratin 8 polyclonal antibody

Manufacturer	Bioworld Technology, Inc
Category	Neuroscience Neural Lineage Markers Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot Immunofluorescence Immunocytochemistry Primary Antibodies Intermediate filaments
Type	Antibody Polyclonal
Specific against	Human, Mouse, Rat
Applications	WB, IF, IHC, ICC
Amount	50ul
Host	Rabbit
Item no.	BS6058-50
Targets	KRT8
eClass 6.1	32160702
eClass 9.0	32160702
Available
Swiss-Prot	P05787
Application all	WB: 1:500ca.1:2000 IHC: 1:50ca.1:200 ICC/IF: 1:50ca.1:200
Background	Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Product	1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Purification and Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Specificity	Cytokeratin 8 pAb detects endogenous levels of Cytokeratin 8 protein.
WB	WesternBlot (WB) analysis of Cytokeratin 8 polyclonal antibody
IHC	Immunohistochemistry (IHC) analysis of Cytokeratin 8 polyclonal antibody in paraffin-embedded Human Lung Cancer tissue.
IF	Immunofluorescence analysis of Hela cells, using Cytokeratin 8 polyclonal antibody
BiowMW	ca. 55 kDa
Note	For research use only, not for use in diagnostic procedure.
Alternative Name	Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, Short name=K8, Type-II keratin Kb8, KRT8, CYK8
Immunogen	Recombinant full length Human Cytokeratin 8.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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