« Back
Home /
Anti-GTP-binding protein Rheb Rheb Antibody

Anti-GTP-binding protein Rheb Rheb Antibody

Item no.	BOS-A00827-1
Manufacturer	Boster
Amount	0.1 mg
Quantity options	0.1 mg 0.1 mg
Category	Oncology By Cell Type Hematology Leukemia Lymphoma Myeloma Cell Biology Cytoskeleton Immunohistochemistry Western Blot ELISA Immunofluorescence Immunostaining small GTPases Rheb Subfamily
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Alias	RHEB2,RHEB2,GTP-binding protein Rheb,Ras homolog enriched in brain
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Rheb antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	20497 MW
Clonality	Polyclonal
Application Details	Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Gene Name	RHEB
Gene Full Name	Ras homolog enriched in brain
Background	Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
Immunogen	Rheb antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb. The immunogen is located within amino acids 40 - 90 of Rheb.
Contents	Rheb Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	Rheb Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Signal Transduction, Cancer
Protein Name	Ras homolog enriched in brain
Protein Function	Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.
Subcellular Localization	Endomembrane system
Description	Boster Bio Anti-GTP-binding protein Rheb Rheb Antibody (Catalog # A00827-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Predicted Reactivity	Bovine

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A00827-1-datasheet.pdf

BOS-A00827-1-safety-datasheet.pdf