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Anti-PLA2G4A Antibody (Center)

Anti-PLA2G4A Antibody (Center)

Item no.	BOS-A00854-1-80ul
Manufacturer	Boster
Amount	80 ul
Quantity options	400 ul 80 ul
Category	Metabolism Cholesterol & Lipid Metabolism Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Enzymes Fatty Acid Catabolism
Applications	WB, FC, IF, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Alias	Cytosolic phospholipase A2,cPLA2,Phospholipase A2 group IVA,Phospholipase A2,Phosphatidylcholine 2-acylhydrolase,Lysophospholipase,PLA2G4A,CPLA2,PLA2G4
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight	85239 Da
Clonality	Polyclonal
Application Details	IF: 1:10-1:50 WB: 1:1000 IHC-P: 1:10-1:50 FC: 1:10-1:50
Gene Name	PLA2G4A
Background	PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
Immunogen	This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A.
Contents	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Manufacturer - Research Category	Cardiovascular, Immunology, Metabolism, Signal Transduction, Inflammation
Protein Function	Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed:7794891, PubMed:8619991, PubMed:8702602, PubMed:9425121, PubMed:10358058, PubMed:14709560, PubMed:16617059, PubMed:17472963, PubMed:27642067, PubMed:18451993). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (By similarity). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:7794891, PubMed:8619991, PubMed:9425121, PubMed:10358058, PubMed:17472963, PubMed:18451993). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway (PubMed:18451993, PubMed:7794891, PubMed:9425121, PubMed:10358058, PubMed:17472963). In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids (PubMed:27642067). Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific (PubMed:7794891). Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides (PubMed:12672805). Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (PubMed:7794891).
Subcellular Localization	Cytoplasm. Golgi apparatus membrane. Nucleus envelope Note=Translocates to intracellular membranes in a calcium-dependent way.
Description	Boster Bio Anti-PLA2G4A Antibody (Center) (Catalog # A00854-1). Tested in WB, Flow Cytometry, IF, IHC-P application(s). This antibody reacts with Human.
Tissue Specificity	Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level) (PubMed:25102815)
Predicted Reactivity	Chicken, Zebrafish, Horse, Mouse, Rabbit, Rat

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BOS-A00854-1-80ul-datasheet.pdf

BOS-A00854-1-80ul-safety-datasheet.pdf