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Anti-LATS1 Antibody Picoband®

Anti-LATS1 Antibody Picoband®

Item no.	BOS-A01051-2-10ug
Manufacturer	Boster
Amount	10 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Neuroscience Molecular Targets for Neuroscience Antibodies Flow Cytometry Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Primary
Format	Lyophilized
Applications	WB, FC, IHC, ICC, ELISA, IHC-F
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Citations	1. Britschgi, A., Duss, S., Kim, S., Couto, J. P., Brinkhaus, H., Koren, S., De Silva, D., Mertz, K. D., Kaup, D., Varga, Z., Voshol, H., Vissieres, A., and 9 others. The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ER-alpha Nature 541: 541-545, 2017. 2. Kemp, C. J. You don't need a backbone to carry a tumour suppressor gene. Nature Genet. 21: 147-148, 1999.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Serine/threonine-protein kinase LATS1,Large tumor suppressor homolog 1,WARTS protein kinase,h-warts,LATS1
Available
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Molecular Weight	150 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells ELISA, 0.1-0.5μg/ml
Gene Name	LATS1
Gene Full Name	large tumor suppressor kinase 1
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Serine/threonine-protein kinase LATS1 is an enzyme that in humans is encoded by the LATS1 gene. It is mapped to 6q25.1. The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Immunogen	E. coli-derived human LATS1 recombinant protein (Position: Q637-A698).
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05 mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Cell Division, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction
Protein Function	Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668).
Subcellular Localization	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Description	Boster Bio Anti-LATS1 Antibody Picoband® catalog # A01051-2. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Expressed in all adult tissues examined except for lung and kidney.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A01051-2-10ug-datasheet.pdf

BOS-A01051-2-10ug-safety-datasheet.pdf