Anti-SRSF protein kinase 1 SRPK1 Antibody

SRPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Polyclonal

SRPK1

SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.

SRPK1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human SRPK1. The immunogen is located within the first 50 amino acids of SRPK1.

SRPK1 Antibody is affinity chromatography purified via peptide column.

Cell Cycle, Homeostasis

Serine/arginine-rich protein-specific kinase which
specifically phosphorylates its substrates at serine residues
located in regions rich in arginine/serine dipeptides, known as RS
domains and is involved in the phosphorylation of SR splicing
factors and the regulation of splicing. Plays a central role in
the regulatory network for splicing, controlling the intranuclear
distribution of splicing factors in interphase cells and the
reorganization of nuclear speckles during mitosis. Can influence
additional steps of mRNA maturation, as well as other cellular
activities, such as chromatin reorganization in somatic and sperm
cells and cell cycle progression. Isoform 2 phosphorylates SFRS2,
ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a
directional (C-terminal to N-terminal) and a dual-track mechanism
incorporating both processive phosphorylation (in which the kinase
stays attached to the substrate after each round of
phosphorylation) and distributive phosphorylation steps (in which
the kinase and substrate dissociate after each phosphorylation
event). The RS domain of SRSF1 binds first to a docking groove in
the large lobe of the kinase domain of SRPK1. This induces certain
structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing
RRM2 to bind the kinase and initiate phosphorylation. The cycles
continue for several phosphorylation steps in a processive manner
(steps 1-8) until the last few phosphorylation steps
(approximately steps 9-12). During that time, a mechanical stress
induces the unfolding of the beta-4 motif in RRM2, which then
docks at the docking groove of SRPK1. This also signals RRM2 to
begin to dissociate, which facilitates SRSF1 dissociation after
phosphorylation is completed. Isoform 2 can mediate hepatitis B
virus (HBV) core protein phosphorylation. It plays a negative role
in the regulation of HBV replication through a mechanism not
involving the phosphorylation of the core protein but by reducing
the packaging efficiency of the pregenomic RNA (pgRNA) without
affecting the formation of the viral core particles. Isoform 1 and
isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of
isoform 1/isoform 2 plays a decisive role in determining cell fate
in K-562 leukaemic cell line: isoform 2 favors proliferation where
as isoform 1 favors differentiation.

Boster Bio Anti-SRSF protein kinase 1 SRPK1 Antibody (Catalog # A01937-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.