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Anti-ACAT1 Antibody Picoband®

Anti-ACAT1 Antibody Picoband®

Item no.	BOS-A02008-1-Cy3
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Obesity Flow Cytometry Immunohistochemistry Western Blot ELISA Immunofluorescence Immunocytochemistry Enzymes Fatty Acid Synthesis Immunostaining Immunochemistry
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Cy3
Citations	1. Daum, R. S., Scriver, C. R., Mamer, O. A., Delvin, E., Lamm, P. H., Goldman, H. An inherited disorder of isoleucine catabolism causing accumulation of alpha-methylacetoacetate and alpha-methyl-beta-hydroxybutyrate and intermittent metabolic acidosis. Pediat. Res. 7: 149-160, 1973. 2. Fukao, T., Matsuo, N., Zhang, G. X., Urasawa, R., Kubo, T., Kohno, Y., Kondo, N. Single base substitutions at the initiator codon in the mitochondrial acetoacetyl-CoA thiolase (ACAT1/T2) gene result in production of varying amounts of wild-type T2 polypeptide. Hum. Mutat. 21: 587-592, 2003. 3. Fukao, T., Nakamura, H., Nakamura, K., Perez-Cerda, C., Baldellou, A., Barrionuevo, C. R., Castello, F. G., Kohno, Y., Ugarte, M., Kondo, N. Characterization of six mutations in five Spanish patients with mitochondrial acetoacetyl-CoA thiolase deficiency: effects of amino acid substitutions on tertiary structure. Molec. Genet. Metab. 75: 235-243, 2002.
Alias	Bifunctional epoxide hydrolase 2,Cytosolic epoxide hydrolase 2,CEH,3.3.2.10,Epoxide hydratase,Soluble epoxide hydrolase,SEH,Lipid-phosphate phosphatase,3.1.3.76,EPHX2
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Observed Molecular weight	40 kDa
Calculated Molecular weight	62616 MW
Clonality	Polyclonal
Application Details	Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/ml
Gene Name	ACAT1
Gene Full Name	Bifunctional epoxide hydrolase 2
Background	Acetyl-CoA acetyltransferase, mitochondrial, also known as acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT1 (Acetyl-Coenzyme A acetyltransferase 1) gene. This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Immunogen	E.coli-derived human ACAT1 recombinant protein (Position: S10-Q404).
Contents	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cancer Metabolism, Cardiovascular, Cell Biology, Cholesterol Metabolism, Drug Metabolism, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction, Vasculature
Protein Name	Bifunctional epoxide hydrolase 2
Protein Function	Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo- 9, 10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro- 9, 10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy- octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate.
Subcellular Localization	Cytoplasm. Peroxisome.
Description	Boster Bio Anti-ACAT1 Antibody Picoband® catalog # A02008-1. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Ubiquitous. A high level expression is seen in secretory tissues.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A02008-1-Cy3-datasheet.pdf

BOS-A02008-1-Cy3-safety-datasheet.pdf