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Anti-JMJD1A KDM3A Antibody

Anti-JMJD1A KDM3A Antibody

Item no.	BOS-A03994-1
Manufacturer	Boster
Amount	0.1 mg
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Alias	TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, TSGA, Lysine-specific demethylase 3A, JmjC domain-containing histone demethylation protein 2A
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	JMJD1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Observed Molecular Weight	147341 MW
Clonality	Polyclonal
Application Details	WB: 1-2 ?g/mL; IHC: 5 ?g/mL; IF: 20 ?g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Manufacturer - Gene Name	KDM3A
Gene Full Name	Lysine (K)-specific demethylase 3A
Background	The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.
Immunogen	Anti-JMJD1A antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50-100 of JMJD1A.
Contents	JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
Purification	JMJD1A Antibody is affinity chromatography purified via peptide column.
Concentration	1 mg/mL
Manufacturer - Research Category	Stem Cells
Protein Name	Lysine (K)-specific demethylase 3A
Protein Function	Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Subcellular Localization	Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).
Short Description	Boster Bio Anti-JMJD1A KDM3A Antibody (Catalog # A03994-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Rat.
Description	Boster Bio Anti-JMJD1A KDM3A Antibody (Catalog # A03994-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Rat.
Predicted Reactivity	Mouse, Chicken

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-A03994-1-datasheet.pdf

BOS-A03994-1-safety-datasheet.pdf