Anti-Transferrin Purified Azide Free TF Monoclonal Antibody

WB, IHC-P, ICC, ELISA, RIA, Functional Assay

Store at 2-8°C. Do not freeze.

Monoclonal

TF

Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.

Phosphate buffered saline (PBS), pH 7.4, azide-free

1 mg/ml

Transferrins are iron binding transport proteins which
can bind two Fe (3+) ions in association with the binding of an
anion, usually bicarbonate. It is responsible for the transport of
iron from sites of absorption and heme degradation to those of
storage and utilization. Serum transferrin may also have a further
role in stimulating cell proliferation.

Boster Bio Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (Catalog# M00094-1). Tested in WB, IHC-P, ICC, ELISA, RIA, Functional Assay application(s). This antibody reacts with Human, Pig, Rabbit.