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Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody

Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody

Item no.	BOS-M00173-1-30ul
Manufacturer	Boster
Amount	30 ul
Quantity options	100 ul 30 ul
Category	Oncology Antibodies By Cell Type Carcinoma Hematology Lymphoma Melanoma Sarcoma Flow Cytometry Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence Immunocytochemistry Primary Antibodies Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IP, IHC, ICC
Clone	CGA-3
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<50 pg/ml
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Cyclin-dependent kinase inhibitor 1B,Cyclin-dependent kinase inhibitor p27,p27Kip1,CDKN1B,KIP1
Available
Manufacturer - Category	Primary Antibodies, Rabbit Monoclonal Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Molecular Weight	25 kDa
Calculated Molecular weight	22073 MW
Clonality	Monoclonal
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 FC 1:50
Gene Name	CDKN1B
Gene Full Name	Cyclin-dependent kinase inhibitor 1B
Immunogen	A synthesized peptide derived from human p27 KIP 1
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/ml BSA.
Purification	Affinity-chromatography
Reconstitution	Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Concentration	Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling
Protein Name	P2X purinoceptor 1
Protein Function	Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Subcellular Localization	Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).
Description	Boster Bio Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody catalog # M00173-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-M00173-1-30ul-datasheet.pdf

BOS-M00173-1-30ul-safety-datasheet.pdf