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Anti-PBK Antibody Picoband®

Anti-PBK Antibody Picoband®

Item no.	BOS-PB9310-APC
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Oncology Neuroscience Neural Lineage Markers Metabolism Cholesterol & Lipid Metabolism Targets for Rodent Neurosciences Neural Development Antibodies By Cell Type Carcinoma Hematology Myeloma Cell Biology Cell Culture Cytokines Cell Status Apoptosis Cytoskeleton Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Immunocytochemistry Enzymes Fatty Acid Catabolism Primary Antibodies By Organ Lung Cancer Adhesion Molecule Markers Progenitor Markers Immunochemistry small GTPases Arf Subfamily
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	APC
Sensitivity	<5 pg/ml
Sequence	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Citations	1. Abe Y, Matsumoto S, Kito K, Ueda N (Aug 2000). Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells. J Biol Chem 275 (28): 21525–31. 2. Entrez Gene: PBK PDZ binding kinase 3. Park, J.-H., Lin, M.-L., Nishidate, T., Nakamura, Y., Katagiri, T. PDZ-binding kinase/T-LAK cell-originated protein kinase, a putative cancer/testis antigen with an oncogenic activity in breast cancer. Cancer Res. 66: 9186-9195, 2006.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Lymphokine-activated killer T-cell-originated protein kinase,2.7.12.2,Cancer/testis antigen 84,CT84,MAPKK-like protein kinase,Nori-3,PDZ-binding kinase,Spermatogenesis-related protein kinase,SPK,T-LAK cell-originated protein kinase,PBK,TOPK
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	40 kDa
Calculated Molecular weight	36085 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Gene Name	PBK
Gene Full Name	Lymphokine-activated killer T-cell-originated protein kinase
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Lymphokine-activated killer T-cell-originated protein kinase, also known as TOPK or PBK, is an enzyme that in humans is encoded by the PBK gene. The protein encoded by this gene is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is mapped to 8p21.1. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis.
Immunogen	E.coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cell Division, Cytokinesis, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, MAPK Pathway, p53 Pathway, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction
Protein Name	Lymphokine-activated killer T-cell-originated protein kinase
Protein Function	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Subcellular Localization	Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.
Description	Boster Bio Anti-PBK Antibody Picoband® catalog # PB9310. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Sequence Similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Manufacturer - Alias	Lymphokine-activated killer T-cell-originated protein kinase, 2.7.12.2, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-PB9310-APC-datasheet.pdf

BOS-PB9310-APC-safety-datasheet.pdf