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Anti-Nucleobindin 2/NUCB2 Antibody Picoband®

Anti-Nucleobindin 2/NUCB2 Antibody Picoband®

Item no.	BOS-PB9876-Dylight550
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Oncology Neuroscience Metabolism Regulation of Appetite Neural Development Antibodies By Cell Type Blastoma Immunohistochemistry Western Blot ELISA Primary Antibodies By Organ Gastric Cancer Brain Cancer Immunostaining
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, IHC, ELISA
Specific against	Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Dylight 550
Sensitivity	<20 pg/ml
Citations	1. Entrez Gene: NUCB2 nucleobindin 2. 2. Barnikol-Watanabe S, Gross NA, Gotz H, Henkel T, Karabinos A, Kratzin H, Barnikol HU, Hilschmann N (February 1995). Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein. Biol Chem Hoppe Seyler 375 (8): 497–512. 3. DeAngelis, M., Drury, S., Doucet, J., Sherry, S., Buckley, L., Pelias, M., Deininger, P., Keats, B., Batzer, M. Construction of a BAC contig and isolation of candidate genes in the Acadian Usher syndrome (USH1C) critical region. (Abstract) Am. J. Hum. Genet. 61 (suppl.): A233 only, 1997.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Nucleobindin-2,DNA-binding protein NEFA,Prepronesfatin,Nesfatin-1,Nucb2,Nefa
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	55 kDa
Calculated Molecular weight	50304 MW
Clonality	Polyclonal
Application Details	Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, - Western blot, 0.1-0.5μg/ml, Mouse
Gene Name	Nucb2
Gene Full Name	Nucleobindin-2
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Nucleobindin-2?is a?calcium-binding?EF-hand protein?that in humans is encoded by the?NUCB2?gene. By exon trapping and sequence analysis, ?the?NUCB2?sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.
Immunogen	E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Calcium Binding Proteins, Calcium Signaling, Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Signal Transduction, Signaling Pathway
Protein Name	Nucleobindin-2
Protein Function	Calcium-binding protein. May have a role in calcium homeostasis.
Subcellular Localization	Cytoplasm. Perikaryon. Endoplasmic reticulum. Golgi apparatus. Nucleus envelope. Membrane; Peripheral membrane protein. Secreted. In dendrites and perikarya of brain neurons. Abundant in the ER cisternae and nuclear envelope, but not detected in Golgi, mitochondria or nucleoplasm in neurons. In cell culture, cytoplasmic and secreted.
Description	Boster Bio Anti-Nucleobindin 2/NUCB2 Antibody Picoband® catalog # PB9876. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Found in liver, heart, thymus, muscle, intestine, kidney, lung, spleen and throughout the brain, in cerebral cortex, hippocampus, hypothalamus and medulla oblongata. Nucb2 and necdin levels were higher in postmitotic neurons.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PB9876-Dylight550-datasheet.pdf

BOS-PB9876-Dylight550-safety-datasheet.pdf