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Human recombinant TGF beta 1 (Transforming growth factor beta 1) protein, AF

Human recombinant TGF beta 1 (Transforming growth factor beta 1) protein, AF

Item no.	BOS-PROTP01137-10
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug 20 ug 5 ug
Category	Oncology Neuroscience Neuroinflammation By Cell Type Carcinoma Cell Biology Cell Culture Cytokines Mesothelioma By Organ Colorectal Cancer Cell Culture
Format	Lyophilized
Applications	Cell Culture
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Sequence	A DNA sequence encoding Human CD28 ECD (# P01137) (Met1-Ser390) was expressed with polyhistidine tag at the N-terminus.
Alias	Differentiation inhibiting factor,Cartilage-inducing factor,CED,DPD1,IBDIMDE,LAP,TGFB
Available
Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His Tag (N-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 44.3 kDa. The protein migrates as approximately 10、40、50 kDa under reducing condition (SDS-PAGE analysis) corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation
Manufacturer - Format	The protein was lyophilized from a 0.2 µm filtered solution of PBS, pH 7.0. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 44.3 kDa.The protein migrates as approximately 10、40、50 kDa under reducing condition (SDS-PAGE analysis) corresponding to mature TGFβ1, LAP protein and inacitve latent TGFβ1 respectively due to glycosylation
Gene Name	TGFB1
Purification	>95% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile 10 mM HCl to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.
Bioactivity/Biological Activity	Measure by its ability to inhibit the IL-4 dependent proliferation in TF-1 cells. The ED₅₀ for this effect is < 0.1 ng/mL.
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PROTP01137-10-datasheet.pdf

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Amount: 100 ug

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Delivery expected until 12/4/2025

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