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Human recombinant GFAP protein, AF

Human recombinant GFAP protein, AF

Item no.	BOS-PROTP14136
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug 20 ug 5 ug
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Targets for Rodent Neurosciences By Cell Type Blastoma Neurodegeneration Markers Microglia Markers Cell Culture
Format	Lyophilized
Applications	Cell Culture
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Sequence	A DNA sequence encoding Human GFAP Protein (#P14136)(Met1-Met432) was expressed with polyhistidine tag at the C-terminus.
Alias	Glial fibrillary acidic protein
Available
Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His Tag (C-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 51 kDa. The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 51 kDa.The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	GFAP
Purification	>98% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	GFAP (Glial Fibrillary Acidic Protein) is a protein primarily found in CNS astrocytes. Increased GFAP immunoreactivity indicates gliosis, a response to neural damage. GFAP defects cause Alexander disease, a rare CNS disorder with astrocytic Rosenthal fiber accumulation. The infantile form leads to myelination failure and early mortality, while the juvenile or adult forms present with ataxia, bulbar signs, spasticity, progressing more gradually.
Bioactivity/Biological Activity	Testing in process
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PROTP14136-datasheet.pdf

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Amount: 100 ug

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Delivery expected until 12/4/2025

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