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Human recombinant Sonic Hedgehog (C24II) protein, AF

Human recombinant Sonic Hedgehog (C24II) protein, AF

Item no.	BOS-PROTQ15465-6-20ug
Manufacturer	Boster
Amount	20 ug
Quantity options	100 ug 20 ug 5 ug
Category	Oncology Neuroscience Neural Signaling By Cell Type Blastoma Sarcoma Cell Culture
Format	Lyophilized
Applications	Cell Culture
Specific against	Human (Homo sapiens)
Conjugate/Tag	SUMO, HIS
Sequence	IIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
Alias	SHH,TPT,HHG1,HLP3,HPE3,SMMCI,ShhNC,TPTPS,MCOPCB5
Available
Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His-SUMO Tag (N-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 31.86 kDa. The protein migrates as 35-45 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 31.86 kDa.The protein migrates as 35-45 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	SHH
Purification	>95% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	Sonic hedgehog protein (SHH) plays a critical role in development of embryonic morphogenesis which mediate the process of organogenesis and central nervous system. The sonic hedgehog signal pathway also plays an important role in cancerous tumors like embryonic cerebellar tumor, prostate cancer tumours and medulloblastoma. Shh binds the receptor (Ptc1) that induce the smo protein to activate the downstream pathway, which induce the shh target gene expression.
Bioactivity/Biological Activity	Testing in process
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PROTQ15465-6-20ug-datasheet.pdf

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Amount: 20 ug

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Delivery expected until 12/4/2025

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