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Swine recombinant IL-10 (Interleukin-10) protein, AF

Swine recombinant IL-10 (Interleukin-10) protein, AF

Item no.	BOS-PROTQ29055-5ug
Manufacturer	Boster
Amount	5 ug
Quantity options	20 ug 5 ug
Category	Neuroscience Neuroinflammation Cell Biology Cell Culture Cytokines Cell Culture
Format	Lyophilized
Applications	Cell Culture
Specific against	Pig (Porcine, Sus scrofa domesticus)
Conjugate/Tag	HIS
Sequence	MSIKSENSCIHFPTSLPHMLRELRAAFGPVKSFFQTKDQMGDLLLTGSLLEDFKGYLGCQALSEMIQFYLEDVMPKAESDGEDIKEHVNSLGEKLKTLRLRLRRCHQFLPCENKSKAVEEVKSAFSKLQERGVYKAMGEFDIFINYIEAYMTMKMRKN with polyhistidine tag at the C-terminus.
Alias	B-TCGF,CSIF,TGIF
Available
Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His Tag (C-term)
Storage Conditions	Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 19.05 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 19.05 kDa.The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	IL10
Purification	>98% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	Interleukin 10 (IL-10) is a 17.54 kDa cytokine with 158 amino acid residues and is also known as human cytokine synthesis inhibitory factor (CSIF). IL-10 is an anti-inflammatory cytokine that is mainly secreted from monocytes, lymphocytes, and mast cells. IL-10 participates in the survival, proliferation, and antibody production of B cell via the JAK-STAT signaling pathway and suppress pro-inflammatory cytokine secretion by blocking the NF-κB signaling pathway.
Bioactivity/Biological Activity	Measure by its ability to induce proliferation in MC/9‑2 cells. The ED₅₀ for this effect is <5 ng/mL.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5 ug

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Delivery expected until 12/4/2025

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