« Back
Home /
Human recombinant R-spondin 1 protein, AF

Human recombinant R-spondin 1 protein, AF

Item no.	BOS-PROTQ2MKA7-1
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug 20 ug 5 ug
Category	Oncology By Cell Type Carcinoma Cell Culture
Format	Liquid
Applications	Cell Culture
Specific against	Human (Homo sapiens)
Conjugate/Tag	SUMO, HIS
Sequence	A DNA sequence encoding Human R-Spondin 1 Protein (#Q2MKA7) (Ser21 - Ala263) was expressed with polyhistidine-SUMO tag at the N-terminus.
Alias	RSPO1,Roof plate-specific spondin-1
Available
Manufacturer - Category	Recombinant Proteins
Manufacturer - Conjugate / Tag	His-SUMO Tag (N-term)
Storage Conditions	Store at 2°C to 8°C for up to 2 weeks, -20°C or -80°C for 6 months. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Molecular Weight	The protein has a calculated MW of 46.8 kDa. The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).
Manufacturer - Format	Liquid (1X PBS, pH 7.4) If you have any concerns or special requirements, please confirm with us.
Calculated Molecular weight	The protein has a calculated MW of 46.8 kDa.The protein migrates as 62-68 kDa under reducing condition (SDS-PAGE analysis).
Gene Name	RSPO1
Purification	>95% as determined by SDS-PAGE.
Reconstitution	Centrifuge at 3000 rpm for 5 mins before opening. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Description	R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/6), subsequent clearing negative regulators ZNRF3/RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.
Bioactivity/Biological Activity	R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is 1-3 μg/mL.
Endotoxin level	<1 EU per 1 μg of the protein by the LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-PROTQ2MKA7-1-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 100 ug

available

Delivery expected until 12/4/2025

Express Delivery: Save 1 days

Compare

Add to wishlist

Get an offer