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Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)

Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)

Item no.	BOS-MA1049
Manufacturer	Boster
Amount	100 ug/vial
Quantity options	100 ug/vial 10 ug
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Neural Mitochondrial Function Targets for Rodent Neurosciences Antibodies By Cell Type Melanoma Immunohistochemistry Western Blot Primary Antibodies Neurodegeneration Markers
Type	Antibody Monoclonal
Format	Lyophilized
Applications	WB, IHC
Clone	LK1
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Chicken (Gallus gallus domesticus)
Host	Mouse
Isotype	IgG1
Sensitivity	>5000 cells
Sequence	Belongs to the chaperonin (HSP60) family.
Citations	1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Hansen, J. J.; Bross, P.; Westergaard, M.; Nielsen, M. N.; Eiberg, H.; Borglum, A. D.; Mogensen, J.; Kristiansen, K.; Bolund, L.; Gregersen, N. : Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a biional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only, 2003. 3. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	60 kDa heat shock protein,mitochondrial,60 kDa chaperonin,Chaperonin 60,CPN60,HSP-65,Heat shock protein 60,HSP-60,Hsp60,Mitochondrial matrix protein P1,Hspd1,Hsp60
Similar products	Heat shock protein 60, 60 kDa chaperonin, Chaperonin 60, CPN60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HuCHA60, GROEL, SPG13, 60-KD, spastic paraplegia 13(autosomal dominant), CHAPERONIN
Available
Manufacturer - Category	Primary Antibodies, Mouse Monoclonal Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	65 kDa
Calculated Molecular weight	60955 MW
Clonality	Monoclonal
Application Details	Western blot, 2-4μg/ml, Human, chicken, rat Immunohistochemistry (Paraffin-embedded Section), 4-8μg/ml, Human, chicken, mouse, rat
Gene Name	HSPD1
Gene Full Name	60 kDa heat shock protein, mitochondrial
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
Immunogen	Recombinant human heat shock protein 60 (HSP60)
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification	Ascites
Reconstitution	Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Concentration	Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Manufacturer - Research Category	Chaperones, Heat Shock Proteins, Mitochondria, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers, Tags & Cell Markers
Protein Name	60 kDa heat shock protein, mitochondrial
Protein Function	Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Subcellular Localization	Mitochondrion matrix.
Description	Boster Bio Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) catalog # MA1049. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Tissue Specificity	Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles.
Sequence Similarities	Belongs to the chaperonin (HSP60) family.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-MA1049-datasheet.pdf

BOS-MA1049-safety-datasheet.pdf