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Anti-Hsp27/HSPB1 Antibody Picoband™

Anti-Hsp27/HSPB1 Antibody Picoband™

Manufacturer	Boster
Category	Oncology Antibodies Immunohistochemistry Western Blot Primary Antibodies By Organ Brain Cancer
Type	Antibody Polyclonal
Specific against	Human
Isotype	IgG
Format	Lyophilized
Applications	WB, FC, IF, IHC, ICC, IHC-F
Amount	100 ug/vial
Host	Rabbit
Item no.	BOS-PB9237
Targets	CD20
eClass 6.1	32160702
eClass 9.0	32160702
Available
Manufacturers Product Category	Primary Antibodies, Rabbit Polyclonal Antibodies
Manufacturers Research Category	Atherosclerosis, Cancer, Cardiovascular, Chaperones, Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers
Short Description	Boster Bio Anti-Hsp27/HSPB1 Antibody Picoband™ catalog # PB9237. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-Hsp27/HSPB1 Antibody Picoband™ catalog # PB9237. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.
Background	HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Gene Name	HSPB1
Application Details	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, - Flow Cytometry, 1-3μg/mlx10⁶ cells, Human, -
Application Notes	WB: The detection limit for Hsp27 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality	Polyclonal
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
Purification	Immunogen affinity purified.
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Gene Full Name	Heat shock protein beta-1
Molecular Weight	22783 MW
Protein Function	Involved in stress resistance and actin organization.
Subcellular Localization	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Tissue Specificity	Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle..
Protein Name	Heat shock protein beta-1
Sequence Similarities	Belongs to the small heat shock protein (HSP20) family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.