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Anti-NEFH Antibody (Monoclonal, N52)

Anti-NEFH Antibody (Monoclonal, N52)

Item no.	BOS-MA1071
Manufacturer	Boster
Amount	100 ug/vial
Quantity options	100 ug/vial 10 ug
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Immunohistochemistry Western Blot Primary Antibodies By Organ Lung Cancer Neurodegeneration Markers Glial Cell Markers
Type	Antibody Monoclonal
Format	Lyophilized
Applications	WB, IHC
Clone	N52
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG1
Sensitivity	<10 pg/ml
Sequence	Belongs to the intermediate filament family.
Citations	1. Lees, J. F.; Shneidman, P. S.; Skuntz, S. F.; Carden, M. J.; Lazzarini, R. A. : The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it. EMBO J. 7: 1947-1955, 1988. 2. Brownlees, J.; Ackerley, S.; Grierson, A. J.; Jacobsen, N. J. O.; Shea, K.; Anderton, B. H.; Leigh, P. N.; Shaw, C. E.; Miller, C. C. J. : Charcot-Marie-Tooth disease neurofilament mutations disrupt neurofilament assembly and axonal transport. Hum. Molec. Genet. 11: 2837-2844, 2002.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Neurofilament heavy polypeptide,NF-H,200 kDa neurofilament protein,Neurofilament triplet H protein,Nefh,Nfh
Similar products	Neurofilament triplet H protein, 200 kDa neurofilament protein, KIAA0845, NFH
Available
Manufacturer - Category	Primary Antibodies, Mouse Monoclonal Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	200 kDa
Calculated Molecular weight	115378 MW
Clonality	Monoclonal
Application Details	Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, - Western blot, 0.5ml, Human, mouse, rat
Gene Name	NEFH
Gene Full Name	Neurofilament heavy polypeptide
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).
Immunogen	C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Purification	Ascites
Reconstitution	Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Concentration	Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Manufacturer - Research Category	Alzheimer's Disease, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction
Protein Name	Neurofilament heavy polypeptide
Protein Function	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Subcellular Localization	Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Description	Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Muscle specific. Located in the thin filament of striated muscle.
Sequence Similarities	Belongs to the intermediate filament family.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-MA1071-datasheet.pdf

BOS-MA1071-safety-datasheet.pdf