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Anti-NEFH Antibody (Monoclonal, N52)

Anti-NEFH Antibody (Monoclonal, N52)

Manufacturer	Boster
Category	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Immunohistochemistry Western Blot Primary Antibodies Glial Cell Markers Neurodegeneration Markers;Research Area Oncology By Organ Lung Cancer
Type	Antibody Monoclonal
Specific against	Human, Mouse, Rat
Clone	N52
Isotype	IgG1
Format	Lyophilized
Applications	WB, IHC
Amount	100 ug/vial
Host	Mouse
Item no.	BOS-MA1071
Targets	NEFH
eClass 6.1	32160702
eClass 9.0	42030590
Available
Manufacturers Product Category	Primary Antibodies, Mouse Monoclonal Antibodies
Manufacturers Research Category	Alzheimer's Disease, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction
Short Description	Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Description	Boster Bio Anti-NEFH Antibody (Monoclonal, N52) catalog # MA1071. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Background	Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).
Gene Name	NEFH
Application Details	Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, - Western blot, 0.5ml, Human, mouse, rat
Application Notes	Other applications have not been tested. Optimal dilutions should be determined by end users.
Clonality	Monoclonal
Concentration	Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Immunogen	C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Purification	Ascites
Cross-reactivity	No cross-reactivity with other proteins
Storage	Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Reconstitution	Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Gene Full Name	Neurofilament heavy polypeptide
Molecular Weight	115378 MW
Protein Function	Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Protein Name	Neurofilament heavy polypeptide
Sequence Similarities	Belongs to the intermediate filament family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.